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KMID : 0624620150480010054
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BMB Reports
2015 Volume.48 No. 1 p.54 ~ p.59
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Autocrine stimulation of IL-10 is critical to the enrichment of IL-10-producing CD40hiCD5+ regulatory B cells in vitro and in vivo
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Kim Hyuk-Soon
Lee Jun-Ho
Han Hee-Dong
Kim A-Ram
Nam Seung-Taek
Kim Hyun-Woo
Park Young-Hwan
Lee Da-Jeong
Lee Min-Bum
Park Yeong-Min
Kim Hyung-Sik
Kim Young-Mi
You Ji-Chang
Choi Wahn-Soo
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Abstract
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IL-10-producing B (Breg) cells regulate various immune responses. However, their phenotype remains unclear. CD40 expression was significantly increased in B cells by LPS, and the Breg cells were also enriched in CD40hiCD5+ B cells. Furthermore, CD40 expression on Breg cells was increased by IL-10, CD40 ligand, and B cell-activating factor, suggesting that CD40hi is a common phenotype of Breg cells. LPS-induced CD40 expression was largely suppressed by an anti-IL-10 receptor antibody and in IL-10?/?CD5+CD19+ B cells. The autocrine effect of IL-10 on the CD40 expression was largely suppressed by an inhibitor of JAK/STAT3. In vivo, the LPS treatment increased the population of CD40hiCD5+ Breg cells in mice. However, the population of CD40hiCD5+ B cells was minimal in IL-10?/? mice by LPS. Altogether, our findings show that Breg cells are largely enriched in CD40hiCD5+ B cells and the autocrine effect of IL-10 is critical to the formation of CD40hiCD5+ Breg cells.
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KEYWORD
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Autocrine, CD5, CD40, Interleukin-10, Regulatory B cells
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